Cassimeris L
Department of Molecular Biology, Lehigh University, Bethlehem, Pennsylvania 18015.
Cell Motil Cytoskeleton. 1993;26(4):275-81. doi: 10.1002/cm.970260402.
Characterization of microtubule assembly in vivo and in vitro has raised questions of how cells regulate dynamic instability. While dynamic instability is an intrinsic property of the tubulin molecule, factors are required to increase the plus-end elongation rate and increase the frequencies of both catastrophe and rescue to achieve cellular tubulin turnover rates. It is likely that the activities of the transition frequency regulators are themselves regulated during the cell cycle, but the mechanisms of regulation are not known. As we identify the proteins which alter microtubule assembly, new classes of MAPs will emerge. An understanding of how these proteins function may provide further insight into how cells organize the different arrays of microtubules used for such processes as vesicle transport, polarized organization of organelles, and chromosome movement.
体内和体外微管组装的特性引发了关于细胞如何调节动态不稳定性的问题。虽然动态不稳定性是微管蛋白分子的固有特性，但需要一些因素来提高正端延伸率，并增加灾难和拯救的频率，以实现细胞微管蛋白的周转率。很可能转换频率调节因子的活性在细胞周期中自身受到调节，但其调节机制尚不清楚。随着我们鉴定出改变微管组装的蛋白质，新的微管相关蛋白（MAPs）类别将会出现。了解这些蛋白质的功能可能会进一步深入了解细胞如何组织用于诸如囊泡运输、细胞器的极化组织和染色体移动等过程的不同微管阵列。
