Sychrová H, Chevallier M R
Department of Membrane Transport, AVCR, Prague, Czech Republic.
Curr Genet. 1993 Dec;24(6):487-90. doi: 10.1007/BF00351710.
Using a gene bank of C. albicans, the lysine-permease deficiency in a strain of S. cerevisiae was complemented, and the restriction map of the corresponding C. albicans DNA fragment was constructed. Its expression in S. cerevisiae showed that the permease of C. albicans actively transports arginine (KT = 18 mumol/l, Jmax = 26 nmol/min per mg dry weight), lysine (KT = 12 mumol/l, Jmax = 18 nmol/min per mg dry weight), histidine (KT = 37 mumol/l, Jmax = 9.7 nmol/min per mg dry weight), as well as their toxic analogues canavanine and thialysine, with high affinity. The intracellular concentration of basic amino acids transported into S. cerevisiae by the C. albicans permease reaches more than a thousand-times-higher value compared to the external concentration in the medium. Accumulated amino acids do not leave the cells. The uptake is strongly reduced by the protonophores and inhibitors of plasma membrane H(+)-ATPase.
利用白色念珠菌的基因文库，对酿酒酵母菌株中的赖氨酸通透酶缺陷进行了互补，并构建了相应白色念珠菌DNA片段的限制性图谱。其在酿酒酵母中的表达表明，白色念珠菌的通透酶能以高亲和力主动转运精氨酸（转运常数KT = 18 μmol/l，最大转运速率Jmax = 每毫克干重26 nmol/min）、赖氨酸（KT = 12 μmol/l，Jmax = 每毫克干重18 nmol/min）、组氨酸（KT = 37 μmol/l，Jmax = 每毫克干重9.7 nmol/min）以及它们的毒性类似物刀豆氨酸和硫赖氨酸。通过白色念珠菌通透酶转运到酿酒酵母中的碱性氨基酸的细胞内浓度比培养基中的外部浓度高出一千多倍。积累的氨基酸不会离开细胞。质子载体和质膜H(+) - ATP酶抑制剂会强烈降低摄取量。
