Revuelta J L, Jayaram M
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7344-7. doi: 10.1073/pnas.83.19.7344.
The fungus, Phycomyces blakesleeanus, shows many well-defined responses to a number of external stimuli. Genetic analysis shows that at least eight genes are involved in Phycomyces sensory transduction. As a first step toward the molecular analysis of these genes and their products, we have developed a transformation protocol for Phycomyces by using a plasmid containing the kanamycin-resistance gene from Tn903 and a Phycomyces DNA fragment capable of supporting autonomous replication in yeast (ARS). Our results demonstrate that the Tn903 gene is expressed in Phycomyces and that the ARS fragment selected in yeast supports autonomous replication in Phycomyces as well.
布氏毛霉这种真菌对多种外部刺激表现出许多明确的反应。遗传分析表明，至少有八个基因参与了布氏毛霉的感觉传导。作为对这些基因及其产物进行分子分析的第一步，我们利用一个含有来自Tn903的卡那霉素抗性基因的质粒和一个能够在酵母中支持自主复制的布氏毛霉DNA片段（ARS），开发了一种布氏毛霉的转化方案。我们的结果表明，Tn903基因在布氏毛霉中表达，并且在酵母中选择的ARS片段在布氏毛霉中也支持自主复制。
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