Yasuda T, Sawazaki K, Nadano D, Takeshita H, Nakanaga M, Kishi K
Department of Legal Medicine, Fukui Medical School, Japan.
Clin Chim Acta. 1993 Sep 17;218(1):5-16. doi: 10.1016/0009-8981(93)90217-r.
Deoxyribonuclease I (DNase I) was purified from the semen of a 38-year-old male and then characterized. The catalytic properties of the purified enzyme closely resembled those of DNase I purified from the urine of this individual and the following other similarities were observed: molecular masses, iodoacetic acid inactivation kinetics, desialylated isoenzyme patterns. However, the behavior of the purified enzymes determined on several different lectin-affinity chromatography columns differed, which suggests that organ-specific glycosylation of DNase I occurs. Multiple forms of the purified seminal DNase I were demonstrated, each of which had a different pI value separated by isoelectric focusing, which is compatible with the reported existence of genetic polymorphism of seminal DNase I (Sawazaki et al., Forensic Sci Int 1992;57:39-44). Furthermore, enzymological and immunological comparisons of purified seminal and urinary and partially purified prostatic DNases I indicated that the prostate may be one of seminal enzyme source tissues.
从一名38岁男性的精液中纯化出脱氧核糖核酸酶I（DNase I）并进行了特性鉴定。纯化酶的催化特性与从该个体尿液中纯化出的DNase I非常相似，并且观察到以下其他相似之处：分子量、碘乙酸失活动力学、去唾液酸化同工酶模式。然而，在几种不同的凝集素亲和色谱柱上测定的纯化酶的行为有所不同，这表明DNase I存在器官特异性糖基化。已证明纯化的精液DNase I有多种形式，通过等电聚焦分离，每种形式都有不同的pI值，这与报道的精液DNase I存在遗传多态性相符（泽崎等人，《法医学国际》1992年；57:39 - 44）。此外，对纯化的精液、尿液和部分纯化的前列腺DNase I进行的酶学和免疫学比较表明，前列腺可能是精液酶的来源组织之一。
