Roisman F R, Castello A, Fainboim H, Morelli A, Fainboim L
Laboratorio de Inmunogenética, Hospital de Clínicas, Argentina.
Clin Immunol Immunopathol. 1994 Feb;70(2):99-103. doi: 10.1006/clin.1994.1016.
We studied the expression of surface (HBsAg) and core (HBcAg) proteins of hepatitis B virus (HBV) on the surface of peripheral blood mononuclear cells (PBMC) from HBV-infected patients. A total of 122 patients with different liver viral diseases was analyzed by indirect immunofluorescence with monoclonal antibodies. The 35 patients with HBV chronic active hepatitis (CAH) and 38 of 60 patients with acute hepatitis B (63%) expressed HBsAg on the PBMC. No expression was detected on the cells from both normal and HBV-unrelated viral hepatitis control groups. Serial follow-up of patients with acute hepatitis B showed that HBsAg expression by PBMC tended to be undetectable 4 months after the onset of the disease and at the same time the clinical improvement was evident. Cell cultures of EBV-transformed B lymphocytes were established from PBMC of HBV-infected patients; immunoelectron microscopy demonstrated the HBsAg on the cellular membrane. One-third of HBV-infected patients who were studied showed the expression of HBcAg by PBMC. HBcAg was detected in patients with acute hepatitis B at the early stage of infection. The cells of these patients also expressed HBsAg in PBMC. In CAH patients, a positive association was observed between the expression of HBcAg and the presence of serum HBeAg.
我们研究了乙型肝炎病毒（HBV）表面蛋白（HBsAg）和核心蛋白（HBcAg）在HBV感染患者外周血单个核细胞（PBMC）表面的表达情况。采用单克隆抗体间接免疫荧光法对总共122例患有不同肝脏病毒性疾病的患者进行了分析。35例慢性活动性乙型肝炎（CAH）患者以及60例急性乙型肝炎患者中的38例（63%），其PBMC上表达HBsAg。在正常对照组和与HBV无关的病毒性肝炎对照组的细胞上均未检测到表达。对急性乙型肝炎患者的系列随访显示，疾病发作4个月后PBMC上的HBsAg表达往往难以检测到，与此同时临床改善明显。从HBV感染患者的PBMC建立了EB病毒转化的B淋巴细胞的细胞培养物；免疫电子显微镜显示细胞膜上有HBsAg。在接受研究的HBV感染患者中，三分之一显示PBMC表达HBcAg。在急性乙型肝炎感染早期的患者中检测到HBcAg。这些患者的细胞在PBMC中也表达HBsAg。在CAH患者中，观察到HBcAg表达与血清HBeAg的存在之间呈正相关。
