鹌鹑（日本鹌鹑）体内的酯酶。物理化学及发育方面的研究。

Esterases in quail (Coturnix coturnix). Physicochemical and developmental aspects.

Tsitsiloni O E, Veini M, Haritos A A

Zoological Laboratory, University of Athens, Faculty of Science, Panepistimiopolis, Greece.

Comp Biochem Physiol B. 1993 Dec;106(4):1009-14. doi: 10.1016/0305-0491(93)90065-d.

Abstract

Soluble esterases of digestive system organs of various developmental stages in the quail (Coturnix coturnix) were resolved by polyacrylamide gel electrophoresis into several molecular forms which were characterized as carboxylesterases, acetylesterases, cholinesterases and esterases sensitive to eserine. 2. The pI of the majority of esterasic activity in several quail and chicken tissues was observed in the range of 5.1-5.6, while the apparent molecular weight in liver extracts was 60,000. 3. The expression of the esterase multiple molecular forms was found to be both tissue- and developmental stage-specific, with electrophoretic patterns becoming more complex in number and/or staining intensity upon hatching and thereafter, especially in liver and intestine.

摘要

通过聚丙烯酰胺凝胶电泳，鹌鹑（Coturnix coturnix）不同发育阶段消化系统器官的可溶性酯酶被分离为几种分子形式，这些分子形式被鉴定为羧酸酯酶、乙酰酯酶、胆碱酯酶和对毒扁豆碱敏感的酯酶。2. 在几种鹌鹑和鸡组织中，大多数酯酶活性的pI值在5.1 - 5.6范围内，而肝脏提取物中表观分子量为60,000。3. 发现酯酶多种分子形式的表达具有组织和发育阶段特异性，孵化后电泳图谱在数量和/或染色强度上变得更加复杂，尤其是在肝脏和肠道中。