鱼类（竹荚鱼）酯酶多种分子形式的物理化学特性及组织分布

Physicochemical characterization and tissue distribution of multiple molecular forms of fish (Trachurus trachurus) esterases.

Salamastrakis S S, Haritos A A

Zoological Laboratory, Faculty of Science, University of Athens, Greece.

Comp Biochem Physiol B. 1988;91(4):741-50. doi: 10.1016/0305-0491(88)90202-7.

Abstract

Tissue-specific electrophoretic patterns of multiple molecular forms of esterases were observed in fish Trachurus trachurus. They were composed of three (in intestine) to nine (in liver) bands characterized mainly as carboxylesterases and acetylesterases. 2. Two major esterase activity bands of pI 4.60 and 4.77 were accompanied by minor ones of higher pH in tissue extracts. 3. Under optimum assay conditions, liver was the richest source of esterase activity followed by intestine, stomach, brain, red muscles, heart, white muscles and gills. 4. Esterases of 70,000 and 420,000 mol. wt were resolved by gel-filtration in liver, intestine and brain. Low size esterases prevailed in liver while the opposite was the case in brain.

摘要

在竹荚鱼中观察到酯酶多种分子形式的组织特异性电泳图谱。它们由三（在肠道中）到九（在肝脏中）条带组成，主要特征为羧酸酯酶和乙酰酯酶。2. 在组织提取物中，两个主要的酯酶活性带，其等电点分别为4.60和4.77，同时伴有较高pH值的次要带。3. 在最佳测定条件下，肝脏是酯酶活性最丰富的来源，其次是肠道、胃、脑、红色肌肉、心脏、白色肌肉和鳃。4. 通过凝胶过滤在肝脏、肠道和脑中分离出分子量为70,000和420,000的酯酶。低分子量酯酶在肝脏中占优势，而在脑中情况则相反。