Prat L, Torres G, Carrió I, Roca M, Riambau V, Berná L, Estorch M, Ferrer I, García C
Nuclear Medicine Department, Hospital de Sant Pau, Barcelona, Spain.
Eur J Nucl Med. 1993 Dec;20(12):1141-5. doi: 10.1007/BF00171011.
To test iodine-125 labelled low-density lipoprotein (125I-LDL), polyclonal indium-111 labelled immunoglobulin G (111In-IgG) and iodine-125 labelled endothelin-1 uptake in metabolically active atheromatous plaques after arterial wall injury, we performed balloon de-endothelialization of carotid arteries or abdominal aortas in 24 New Zealand male rabbits which were fed with a normal diet (n = 14) or a hypercholesterolaemic diet (n = 10) after surgery. Six weeks later the animals were injected with 200 microCi of 125I-LDL and/or with 100 microCi of 111In-IgG or with 9 microCi of 125I-endothelin-1. Forty-eight hours later the animals were sacrificed. Carotid arteries and aortas were removed, counted and fixed for autoradiography and light microscopy examination. Contralateral carotid arteries and thoracic aortas served as controls. Significant 111In-IgG uptake was observed in the injured arteries at autoradiography, with localization mainly in the healing edges, and at well counting. The percentage of the injected dose per gram (%D.inj/g) was 0.0188 +/- 0.06 versus 0.0059 +/- 0.003 in controls (P < 0.05). There was no difference in 111In-IgG uptake between arteries with injury alone and those with active atheroma formation at the site of the injury. Significant 125I-LDL uptake was observed only when lipid deposition was present at light microscopy (%D.inj/g of 0.0024 +/- 0.0005 vs. 0.0010 +/- 0.0003 in controls, P < 0.05). 125I-endothelin-1 accumulation was observed in four of five injured aortas both at autoradiography, with diffuse localization, and at well counting (%D.inj/g of 0.0012 +/- 0.0004 in the abdominal aortas vs 0.0008 +/- 0.0003 in the thoracic aortas).(ABSTRACT TRUNCATED AT 250 WORDS)
为检测碘-125标记的低密度脂蛋白（¹²⁵I-LDL）、多克隆铟-111标记的免疫球蛋白G（¹¹¹In-IgG）和碘-125标记的内皮素-1在动脉壁损伤后代谢活跃的动脉粥样硬化斑块中的摄取情况，我们对24只新西兰雄性兔的颈动脉或腹主动脉进行了球囊去内皮术，术后这些兔子分别喂食正常饮食（n = 14）或高胆固醇饮食（n = 10）。六周后，给动物注射200微居里的¹²⁵I-LDL和/或100微居里的¹¹¹In-IgG或9微居里的¹²⁵I-内皮素-1。48小时后处死动物。取出颈动脉和主动脉，计数并固定用于放射自显影和光学显微镜检查。对侧颈动脉和胸主动脉作为对照。在放射自显影时，在损伤动脉中观察到¹¹¹In-IgG有明显摄取，主要定位于愈合边缘，计数结果也显示如此。每克注射剂量的百分比（%D.inj/g）在对照组为0.0059±0.003，在损伤动脉中为0.0188±0.06（P < 0.05）。仅在损伤处有脂质沉积时才观察到¹²⁵I-LDL有明显摄取（每克注射剂量的百分比在对照组为0.0010±0.0003，有脂质沉积时为0.0024±0.0005，P < 0.05）。在五只损伤的主动脉中，有四只在放射自显影时观察到¹²⁵I-内皮素-1有积聚，呈弥漫性定位，计数结果也显示如此（腹主动脉每克注射剂量的百分比为0.0012±0.0004，胸主动脉为0.0008±0.0003）。（摘要截断于250字）
