Fischman A J, Rubin R H, Khaw B A, Kramer P B, Wilkinson R, Ahmad M, Needelman M, Locke E, Nossiff N D, Strauss H W
Department of Radiology, Massachusetts General Hospital, Boston 02114.
J Nucl Med. 1989 Jun;30(6):1095-100.
The utility of nonspecific polyclonal IgG for external imaging of experimental atherosclerosis was tested in a series of rabbits after balloon catheter deendothelialization of the abdominal aorta. Following injection of 111In-IgG, 111In-Fc, or 111In-Fab serial images were recorded. In addition, several animals received 125I-low density lipoproteins [125I-LDL], or 125I human serum albumin [125I-HSA] as positive and negative controls. Forty-eight hours after injection of the radiolabeled proteins, the aortas were removed, divided into abdominal and thoracic regions, counted, and autoradiographed. The images acquired after injection of 111In-IgG and 111In-Fc, showed clear focal accumulation of radioactivity in the healing abdominal aorta. In contrast, the images obtained after injection of 111In-Fab did not show focal radionuclide accumulation. For 111In-IgG and 111In-Fc there were three to six times as many counts in the abdominal as in the thoracic aorta, while for 111In-Fab and 125I HSA, the abdominal and thoracic counts were nearly equal. The results suggest that radiolabeled IgG and Fc can be used to image experimental atherosclerosis.
在一系列兔腹主动脉球囊导管去内皮化后，测试了非特异性多克隆IgG用于实验性动脉粥样硬化外部成像的效用。注射111铟标记的IgG、111铟标记的Fc或111铟标记的Fab后，记录连续图像。此外，几只动物接受了125碘低密度脂蛋白[125I-LDL]或125碘人血清白蛋白[125I-HSA]作为阳性和阴性对照。注射放射性标记蛋白48小时后，取出主动脉，分为腹段和胸段，计数并进行放射自显影。注射111铟标记的IgG和111铟标记的Fc后获得的图像显示，愈合中的腹主动脉有明显的放射性局灶性聚集。相比之下，注射111铟标记的Fab后获得的图像未显示放射性核素局灶性聚集。对于111铟标记的IgG和111铟标记的Fc，腹主动脉的计数是胸主动脉的三到六倍，而对于111铟标记的Fab和125I-HSA，腹段和胸段的计数几乎相等。结果表明，放射性标记的IgG和Fc可用于实验性动脉粥样硬化的成像。
