Schnorf M, Potrykus I, Neuhaus G
Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich.
Exp Cell Res. 1994 Feb;210(2):260-7. doi: 10.1006/excr.1994.1038.
Five tubing types with different outer and inner diameter dimensions were used to pull injection capillaries with different openings. The correlation between threshold bubble pressure and tip inner and outer diameter was established for each type of tubing. Statistical analysis revealed that the bubble pressure is an accurate measure for the tip inner diameter independent of the tubing used to pull the capillary. A graph directly relating the tip inner diameter to the threshold bubble pressure is presented. The tip outer diameter could not be related to the bubble pressure for tubings of different size. This diameter depends on the wall thickness of the tubing and the puller configuration. Data on the inner tip diameter were used to test the relationship between volume released from injection capillaries and their tip radius. It was found that the volume increases with the fourth power of the radius. Strategies for optimizing the injection capillary are discussed.
使用了五种具有不同外径和内径尺寸的管材来拉制具有不同开口的注射毛细管。针对每种管材，建立了阈值气泡压力与尖端内径和外径之间的相关性。统计分析表明，气泡压力是尖端内径的准确度量，与用于拉制毛细管的管材无关。给出了直接将尖端内径与阈值气泡压力相关联的图表。对于不同尺寸的管材来说，尖端外径与气泡压力无关。该直径取决于管材的壁厚和拉拔器配置。利用尖端内径数据来测试注射毛细管释放的体积与其尖端半径之间的关系。发现体积随半径的四次方增加。讨论了优化注射毛细管的策略。
