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Precise, easy measurement of glass pipet tips for microinjection or electrophysiology.

作者信息

Hagag N, Viola M, Lane B, Randolph J K

机构信息

Dept. of Oncology, State University of New York, Stony Brook 11794.

出版信息

Biotechniques. 1990 Oct;9(4):401-6.

PMID:2257138
Abstract

We describe a simple procedure to measure precisely the tip diameter of glass micropipets. This procedure can improve the precision of microinjection and some electrophysiological measurements due to their extreme sensitivity to the pipet's exact geometric dimensions. The technique is nondestructive and readily carried out as a brief, extra step in these applications. Digital measurements of the threshold pressures for gas bubbling from (gas filled) pipets submerged in methanol were used to calculate the corresponding inner tip diameters using the LaPlace equation. Direct measurements of the inner tip diameters (from 0.2-5.0 mu) using scanning electron microscopy confirmed the validity of this indirect, simple procedure.

摘要

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