超氧阴离子（O2.-）在羟基自由基（HO.）生成中的作用：体外和体内研究

The role of O2.- in the production of HO.: in vitro and in vivo.

作者信息

Liochev S I, Fridovich I

机构信息

Institute of Physiology, Bulgarian Academy of Sciences, Sofia.

出版信息

Free Radic Biol Med. 1994 Jan;16(1):29-33. doi: 10.1016/0891-5849(94)90239-9.

DOI:10.1016/0891-5849(94)90239-9

PMID:8299992

Abstract

In vitro O2.- reduces Fe(III) to Fe(II), which, in turn, reduces the H2O2, yielding Fe(II)O or HO.. In vivo O2.- increases the supply of free iron by oxidatively attacking the [4Fe-4S] clusters of dehydratases such that they release Fe(II), which can then reduce H2O2. In vivo, O2.- also increases the production of H2O2 by acting as an oxidant toward the dehydratases and toward other cellular reductants.

摘要

在体外，超氧阴离子（O₂⁻）将三价铁（Fe(III)）还原为二价铁（Fe(II)），而二价铁又会还原过氧化氢（H₂O₂），生成氢氧化铁（Fe(II)O）或羟基自由基（HO·）。在体内，超氧阴离子通过氧化攻击脱水酶的[4Fe-4S]簇，增加游离铁的供应，使它们释放出二价铁，然后二价铁可以还原过氧化氢。在体内，超氧阴离子还通过作为脱水酶和其他细胞还原剂的氧化剂，增加过氧化氢的产生。