Harris D T, Jaso-Friedmann L, Evans D L
Department of Microbiology and Immunology, University of Arizona, Tucson 85721.
Immunol Lett. 1993 Sep;38(1):11-8. doi: 10.1016/0165-2478(93)90112-f.
Monoclonal antibodies (mAbs) have been derived which identify a target cell antigen involved in human natural killer (NK) cell lysis. The effects of the mAbs on the NK-like cytotoxic activity exhibited by different populations of human cytolytic T cells were examined. The anti-target cell mAbs 1E7 and 18C2 inhibited the lysis of K562 target cells by endogenous NK cells, antigen-specific cytotoxic T lymphocytes (CTL) with NK-like activity, and non-major histocompatibility complex (MHC)-restricted T cells. Cytolysis of K562 target cells was not affected by treatment of the target cells with the anti-class I HLA mAb W6/32. Further, the anti-target cell mAbs had no effect on antigen-specific lysis by the CTL. The mAb W6/32, however, inhibited the antigen-specific killing. Experiments at the single-cell level revealed that the anti-target cell mAbs inhibited the formation of conjugates between the effector cells and K562 tumor cells but had no effect on CTL binding to the antigen-specific target cells. Thus, antigen-specific CTL exhibiting NK-like lytic function appeared to recognize a novel target cell antigen that is distinct from typical MHC antigens and is identical to the target-cell antigen recognized by true NK cells.
已获得单克隆抗体（mAb），其可识别参与人类自然杀伤（NK）细胞裂解的靶细胞抗原。研究了这些单克隆抗体对不同人群的细胞溶解性T细胞所表现出的NK样细胞毒性活性的影响。抗靶细胞单克隆抗体1E7和18C2可抑制内源性NK细胞、具有NK样活性的抗原特异性细胞毒性T淋巴细胞（CTL）以及非主要组织相容性复合体（MHC）限制性T细胞对K562靶细胞的裂解。用抗I类HLA单克隆抗体W6/32处理靶细胞，对K562靶细胞的细胞溶解没有影响。此外，抗靶细胞单克隆抗体对CTL的抗原特异性裂解没有作用。然而，单克隆抗体W6/32可抑制抗原特异性杀伤。单细胞水平的实验表明，抗靶细胞单克隆抗体可抑制效应细胞与K562肿瘤细胞之间结合物的形成，但对CTL与抗原特异性靶细胞的结合没有影响。因此，表现出NK样裂解功能的抗原特异性CTL似乎识别一种新型靶细胞抗原，该抗原不同于典型的MHC抗原，与真正的NK细胞识别的靶细胞抗原相同。
