来自感染牛巴贝斯虫红细胞的糖脂抗原的分离、初步表征及血清学研究。

Isolation, initial characterization and serological studies of glycolipid antigens from Babesia bovis-infected erythrocytes.

作者信息

Orinda G O, Waltisbuhl D J, Rode-Bramanis K, Goodger B V

机构信息

Department of Parasitology, University of Queensland, St. Lucia, Australia.

出版信息

Int J Parasitol. 1993 Dec;23(8):1011-7. doi: 10.1016/0020-7519(93)90121-e.

DOI:10.1016/0020-7519(93)90121-e

PMID:8300296

Abstract

Serological analysis of Babesia bovis-derived glycolipids by ELISA and the indirect fluorescent antibody technique demonstrated the existence of their antigenic and immunogenic activities not only in B. bovis but also in B. bigemina infections. This indicates that serological cross-reactivity of B. bovis and B. bigemina relates to glycolipids. The negative ELISA reaction obtained with Anaplasma marginale antisera suggested the specificity of the reaction to the genus Babesia. Fractionation of these glycolipids by Florisil Sep-Pak column chromatography with subsequent HPTLC immunostaining and Orcinol staining suggested the presence of carbohydrate antigenic determinants in B. bovis glycolipids.

摘要

通过酶联免疫吸附测定（ELISA）和间接荧光抗体技术对牛巴贝斯虫衍生的糖脂进行血清学分析，结果表明，其抗原活性和免疫活性不仅存在于牛巴贝斯虫感染中，也存在于双芽巴贝斯虫感染中。这表明牛巴贝斯虫和双芽巴贝斯虫的血清学交叉反应与糖脂有关。用边缘无形体抗血清获得的阴性ELISA反应表明该反应对巴贝斯虫属具有特异性。通过弗罗里硅土Sep-Pak柱色谱法对这些糖脂进行分离，随后进行高效薄层层析免疫染色和苔黑酚染色，提示牛巴贝斯虫糖脂中存在碳水化合物抗原决定簇。