Allred D R, Ahrens K P
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611-0880.
J Parasitol. 1993 Apr;79(2):274-7.
Intracellular protozoan parasites induce numerous alterations in the invaded host cell, including antigenic modifications of the host cell plasma membrane. We have developed a quantifiable, non-subjective assay for the detection of novel antigenic reactivities on the host cell surface using as a model system bovine erythrocytes infected with Babesia bovis. Infected erythrocytes, metabolically labeled with L-[35S]methionine, were sensitized by incubation with bovine immune serum, then were captured in microtiter plates coated with rabbit anti-bovine IgG antibody. This technique enabled specific capture of B. bovis-infected cells with immune infection sera raised against B. bovis but not with similar sera raised against Babesia bigemina. This assay should be easily applicable to the study of other parasitic diseases.
细胞内原生动物寄生虫会在被入侵的宿主细胞中引发众多变化，包括宿主细胞质膜的抗原修饰。我们已开发出一种可量化、非主观的检测方法，以牛巴贝斯虫感染的牛红细胞作为模型系统，用于检测宿主细胞表面新的抗原反应性。用L-[35S]甲硫氨酸进行代谢标记的感染红细胞，通过与牛免疫血清孵育进行致敏，然后捕获在包被有兔抗牛IgG抗体的微量滴定板中。该技术能够用针对牛巴贝斯虫产生的免疫感染血清特异性捕获感染牛巴贝斯虫的细胞，但不能用针对双芽巴贝斯虫产生的类似血清捕获。这种检测方法应易于应用于其他寄生虫病的研究。
