Hutson R A, Duggleby C J, Lowe J R, Manchee R J, Turnbull P C
Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
J Appl Bacteriol. 1993 Nov;75(5):463-72. doi: 10.1111/j.1365-2672.1993.tb02803.x.
Two DNA probes and a number of oligonucleotide probes were designed from the virulence factor genes of Bacillus anthracis. These probes were tested for specificity against 52 B. anthracis strains and 233 Bacillus strains encompassing 23 other species. A rapid slot blotting technique was used for screening the large numbers of isolates involved. All probes tested appeared to be specific for B. anthracis under high stringency conditions. These probes could differentiate between virulent and avirulent strains. The probes were also applied to the detection of B. anthracis in routine environmental and clinical samples. A non-radioactive hybridization and detection system based on digoxigenin-11-dUTP was developed.
从炭疽芽孢杆菌的毒力因子基因设计了两种DNA探针和一些寡核苷酸探针。对这些探针针对52株炭疽芽孢杆菌菌株以及包括其他23个物种的233株芽孢杆菌菌株进行了特异性测试。采用快速斑点杂交技术对大量分离株进行筛选。在高严格条件下，所有测试的探针似乎对炭疽芽孢杆菌具有特异性。这些探针可以区分有毒力和无毒力的菌株。这些探针还应用于常规环境和临床样本中炭疽芽孢杆菌的检测。开发了一种基于地高辛-11-dUTP的非放射性杂交和检测系统。
