采用库仑检测的高效液相色谱法测定血浆生育酚
Determination of plasma tocopherols by high-performance liquid chromatography with coulometric detection.
作者信息
Sarzanini C, Mentasti E, Vincenti M, Nerva M, Gaido F
机构信息
Department of Analytical Chemistry, University of Torino, Italy.
出版信息
J Chromatogr. 1993 Oct 29;620(2):268-72. doi: 10.1016/0378-4347(93)80015-v.
An HPLC procedure has been developed for tocopherol determination with coulometric detection in human serum samples. Eluent optimization and foreign peak identification (bilirubin) by mass spectrometry are described. An extraction procedure gave yields around 98% with 1.3% coefficient of variation, and the calibration ranged from 0.1 to 200 mg/l with a correlation coefficient of 0.999. The detection limit achieved for vitamin E was 60 pg (3 ng/ml).
已开发出一种用于测定人血清样品中生育酚的高效液相色谱法，并采用库仑检测法。描述了洗脱液的优化以及通过质谱法对杂质峰（胆红素）的鉴定。一种提取方法的回收率约为98%，变异系数为1.3%，校准范围为0.1至200mg/l，相关系数为0.999。维生素E的检测限为60pg（3ng/ml）。
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