Feng Q, Liu K, Hu Z Y, Zou R J, Yang S C, Liu Y X
State Key Laboratory of Reproductive Biology, Chinese Academy of Sciences, Beijing, PRC.
Hum Reprod. 1993 Oct;8(10):1640-4.
Changes of plasminogen activators (PA) during different stages of development of the corpus luteum, and their possible physiological role in luteolysis were studied in rhesus monkeys. It was demonstrated for the first time that monkey corpus luteal cells not only produce PA, but that the function of the corpus luteum is also closely related to the activity of this enzyme system. Generally, the life span for a corpus luteum in monkey is approximately 14-16 days, its demise beginning thereafter. In the present study, we found that urokinase in the corpus luteum is higher on day 5 and day 10 after human chorionic gonadotrophin injection, while the tissue type (t) PA is mainly produced on day 13 when luteolysis may take place. Progesterone production remained high on day 5 and day 10 and decreased dramatically from day 13, indicating the important role of tPA but not urokinase (u) PA in suppressing luteal function. When purified tPA (but not uPA) monoclonal antibody was added to luteal cell culture to neutralize endogenously produced tPA activity, progesterone production in the cells was increased significantly. Interestingly, prolactin alone was capable of increasing PA production by luteal cells; prolactin together with luteinizing hormone, however, had a synergistic luteotrophic effect.
在恒河猴中研究了黄体发育不同阶段纤溶酶原激活剂（PA）的变化及其在黄体溶解中可能的生理作用。首次证明猴黄体细胞不仅产生PA，而且黄体功能也与该酶系统的活性密切相关。一般来说，猴黄体的寿命约为14 - 16天，此后开始衰退。在本研究中，我们发现人绒毛膜促性腺激素注射后第5天和第10天黄体中的尿激酶较高，而组织型（t）PA主要在可能发生黄体溶解的第13天产生。第5天和第10天孕酮分泌仍处于高水平，从第13天开始显著下降，表明tPA而非尿激酶（u）PA在抑制黄体功能中起重要作用。当将纯化的tPA（而非uPA）单克隆抗体添加到黄体细胞培养物中以中和内源性产生的tPA活性时，细胞中的孕酮分泌显著增加。有趣的是，单独的催乳素能够增加黄体细胞的PA分泌；然而，催乳素与促黄体生成素一起具有协同的黄体营养作用。
