Holyoak G R, Giles R C, McCollum W H, Little T V, Timoney P J
Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington 40546-0099.
J Comp Pathol. 1993 Oct;109(3):281-93. doi: 10.1016/s0021-9975(08)80253-8.
The nature and extent of changes associated with equine arteritis virus (EAV) infection of the reproductive tract was documented in 21 prepubertal and 15 peripubertal colts. This study was part of an investigation into the relationship between stage of reproductive tract maturity and susceptibility to the experimental establishment of persistent infection with EAV. After intranasal challenge with a field isolate of EAV, all colts developed clinical signs of equine viral arteritis (EVA) from which they recovered rapidly. Clinical signs during the acute phase consisted of fever, serous to mucopurulent ocular and nasal discharge, oedema of the limbs, scrotum or prepuce, scleral injection, conjunctivitis, icterus, cough, diarrhoea, stiff gait, lethargy, inappetence and depression. At necropsy, the most significant macroscopic lesions included excessive accumulation of fluid within the thoracic and abdominal cavities, lymph node enlargement and oedema of the reproductive tract. Colts killed 7 to 14 days after challenge had acute necrotizing vasculitis involving the testes, epididymides, vasa deferentia, ampullae, prostatic lobes, vesicular glands and bulbourethral glands. Vasculitis was characterized by striking fibrinoid necrosis of small muscular arteries with extravasation of erythrocytes and proteinaceous material into the media, adventitia and perivascular tissues. Colts examined on days 28-180 had lymphocytic and plasmacytic inflammatory cell infiltrates in the lamina propria and muscularis of the epididymides and accessory sex glands. The vascular lesions found during the acute phase of EAV infection contrasted with the multifocal lympho-plasmacytic infiltrates found within the parenchyma of the reproductive tract during the chronic phase. One peripubertal colt was found to be persistently infected with EAV 15 months after challenge. This colt had marked lympho-plasmacytic infiltrates in the ampullae at necropsy.
对21匹青春期前和15匹青春期前后的雄驹进行了记录，以研究与马动脉炎病毒（EAV）生殖道感染相关的变化的性质和程度。本研究是对生殖道成熟阶段与EAV实验性持续性感染易感性之间关系调查的一部分。用EAV的一株野外分离株经鼻攻毒后，所有雄驹均出现马病毒性动脉炎（EVA）的临床症状，但均迅速康复。急性期的临床症状包括发热、浆液性至黏液脓性眼鼻分泌物、四肢、阴囊或包皮水肿、巩膜充血、结膜炎、黄疸、咳嗽、腹泻、步态僵硬、嗜睡、食欲不振和抑郁。尸检时，最显著的宏观病变包括胸腔和腹腔内液体过度积聚、淋巴结肿大和生殖道水肿。攻毒后7至14天处死的雄驹出现急性坏死性血管炎，累及睾丸、附睾、输精管、壶腹、前列腺叶、精囊和尿道球腺。血管炎的特征是小肌性动脉显著的纤维蛋白样坏死，红细胞和蛋白质物质渗入中膜、外膜和血管周围组织。在第28 - 180天检查的雄驹，附睾和附属性腺的固有层和肌层有淋巴细胞和浆细胞炎性细胞浸润。EAV感染急性期发现的血管病变与慢性期生殖道实质内多灶性淋巴细胞和浆细胞浸润形成对比。发现一匹青春期前后的雄驹在攻毒15个月后持续感染EAV。这匹雄驹尸检时壶腹有明显的淋巴细胞和浆细胞浸润。
