Hughes G S, DeSmith V L, Locker P K, Francom S F
Upjohn Research Clinics, Upjohn Company, Kalamazoo, MI 49001.
J Lab Clin Med. 1994 Feb;123(2):290-8.
The purpose of this study was to evaluate the effects of phlebotomy of 500 or 750 ml of whole blood followed by isovolemic hemodilution or autologous transfusion on hemodynamic, hematologic, and biochemical parameters in healthy subjects. Four groups of normovolemic male subjects (n = 6 or 7 per group), aged 18 to 41 years, participated in this 10-day study at Upjohn Research Clinics, Kalamazoo, Mich. On day 1 two groups had phlebotomy of 500 ml; of these, one group underwent immediate postphlebotomy autologous transfusion (group 1) and the other underwent immediate postphlebotomy isovolemic hemodilution and then autologous transfusion on day 3 (group 2). Two other groups had 750 ml phlebotomy, also on day 1; of these, one group underwent immediate postphlebotomy autologous transfusion (group 3) and one underwent immediate postphlebotomy isovolemic hemodilution followed by autologous transfusion on day 3 (group 4). Noninvasive measurement of vital signs, blood pressure, and cardiac function; oximetry; and select hematologic and biochemical parameters were made. On day 1 in groups 2 and 4 transient reductions in hematocrit, hemoglobin, red blood cell count, fibrinogen, and albumin were seen concurrent with prolongation of coagulation studies. Erythropoietin was increased severalfold on day 3 (groups 2 and 4, p < 0.001). The physiologic response to phlebotomy of 500 or 750 ml was similar and was well tolerated in normal subjects.
本研究的目的是评估抽取500或750毫升全血后进行等容血液稀释或自体输血对健康受试者血流动力学、血液学和生化参数的影响。四组血容量正常的男性受试者（每组n = 6或7），年龄在18至41岁之间，参与了在密歇根州卡拉马祖的Upjohn研究诊所进行的这项为期10天的研究。在第1天，两组进行500毫升的静脉放血；其中一组在放血后立即进行自体输血（第1组），另一组在放血后立即进行等容血液稀释，然后在第3天进行自体输血（第2组）。另外两组在第1天也进行750毫升的静脉放血；其中一组在放血后立即进行自体输血（第3组），另一组在放血后立即进行等容血液稀释，然后在第3天进行自体输血（第4组）。对生命体征、血压和心脏功能进行无创测量；进行血氧测定；并检测选定的血液学和生化参数。在第1天，第2组和第4组的血细胞比容、血红蛋白、红细胞计数、纤维蛋白原和白蛋白出现短暂下降，同时凝血研究时间延长。第3天促红细胞生成素增加了几倍（第2组和第4组，p < 0.001）。抽取500或750毫升血液后的生理反应相似，正常受试者耐受性良好。
