Umehara F, Izumo S, Ronquillo A T, Matsumuro K, Sato E, Osame M
Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.
J Neuropathol Exp Neurol. 1994 Jan;53(1):72-7. doi: 10.1097/00005072-199401000-00009.
Immunocytochemical staining of spinal cords from five autopsied patients with HTLV-I-associated myelopathy/tropical spastic paraparesis was performed using a panel of monoclonal or polyclonal antibodies reactive with interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), tumor necrosis factor (TNF)-alpha, interferon (IFN)-alpha, IFN-beta, IFN-gamma and transforming growth factor (TGF)-beta. In the spinal cords of patients with a shorter duration of illness, IL-1 beta, TNF-alpha, and IFN-gamma were expressed on perivascular infiltrating macrophages, astrocytes and microglia in active-chronic inflammatory lesions. In striking contrast, we rarely noted cytokine expression except for IFN-gamma in inactive-chronic lesions of patients with longer durations. In situ expression of these cytokines on microglia and astrocytes, in addition to infiltrating mononuclear cells, suggests that glial cells participate in the inflammatory process, especially in active lesions. In addition, the cytokine expression was gradually downregulated along with duration of illness.
使用一组与白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子（TNF）-α、干扰素（IFN）-α、IFN-β、IFN-γ和转化生长因子（TGF）-β反应的单克隆或多克隆抗体，对5例尸检确诊的成人T细胞白血病病毒I型相关脊髓病/热带痉挛性截瘫患者的脊髓进行免疫细胞化学染色。在病程较短的患者脊髓中，IL-1β、TNF-α和IFN-γ在活动期-慢性炎症病变的血管周围浸润巨噬细胞、星形胶质细胞和小胶质细胞上表达。形成鲜明对比的是，在病程较长患者的非活动期-慢性病变中，除IFN-γ外，我们很少观察到细胞因子表达。这些细胞因子在小胶质细胞、星形胶质细胞以及浸润的单核细胞上的原位表达表明，神经胶质细胞参与了炎症过程，尤其是在活动期病变中。此外，细胞因子表达随着病程逐渐下调。
