[Subtractive hybridization--a technique for extracting DNA sequences, discriminating between two closely-related genomes].
作者信息
Sverdlov E D
出版信息
Mol Gen Mikrobiol Virusol. 1993 Nov-Dec(6):3-12.
PMID:8302314
Abstract
This review deals with an analysis of the current status of genomic DNA subtraction. The theoretical calculations of kinetics of different ways of hybridization are implemented. The main conclusion is that the most preferable strategy for such a subtraction is hybridization of single stranded tracer with single-stranded driver DNA.
摘要
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