通过代表性差异分析从多态性区域分离基因组片段。

Isolation of genomic fragments from polymorphic regions by representational difference analysis.

作者信息

Baldocchi R A, Flaherty L

机构信息

Laboratory of Developmental Genetics, Wadsworth Center, David Axelrod Institute for Public Health, Albany, New York 12201-2002, USA.

出版信息

Methods. 1997 Dec;13(4):337-46. doi: 10.1006/meth.1997.0542.

DOI:10.1006/meth.1997.0542

PMID:9480780

Abstract

Representational difference analysis is an effective technique for isolating the differences between two nearly identical genomes. We have found the technique to be extremely valuable in our analyses of mouse germline mutations. We also present several technical improvements in the procedure that make it more efficient and reliable.

摘要

代表性差异分析是一种用于分离两个几乎相同基因组之间差异的有效技术。我们发现在对小鼠种系突变的分析中，该技术极具价值。我们还在该程序中提出了几项技术改进，使其更高效、更可靠。