Hogg D, Guidos C, Bailey D, Amendola A, Groves T, Davidson J, Schmandt R, Mills G
Toronto Hospital, Ontario, Canada.
Oncogene. 1994 Jan;9(1):89-96.
TTK is a novel protein kinase detectable in all proliferating human cells and tissues. Expression of the TTK gene is markedly reduced or absent in resting cells and in tissues with a low proliferative index. In view of the apparent association between TTK gene expression and cell proliferation, we examined the regulation of this protein kinase during transit of the cell cycle. We found very low levels of TTK mRNA and protein in starved cells. When cells are induced to enter the cell cycle, levels of TTK mRNA, protein and kinase activity increase at the G1/S phase of the cell cycle and peak in G2/M. TTK mRNA levels, as well as kinase activity, drop sharply in early G1, whereas protein levels are largely maintained. TTK may play a role in cell cycle control.
TTK是一种在所有增殖的人类细胞和组织中均可检测到的新型蛋白激酶。在静止细胞和增殖指数较低的组织中，TTK基因的表达显著降低或缺失。鉴于TTK基因表达与细胞增殖之间明显的关联，我们研究了该蛋白激酶在细胞周期进程中的调控情况。我们发现饥饿细胞中TTK mRNA和蛋白的水平非常低。当细胞被诱导进入细胞周期时，TTK mRNA、蛋白和激酶活性在细胞周期的G1/S期增加，并在G2/M期达到峰值。TTK mRNA水平以及激酶活性在G1早期急剧下降，而蛋白水平则基本维持不变。TTK可能在细胞周期调控中发挥作用。
