Masat L, Wabl M, Johnson J P
Institute for Immunology, Ludwig Maximilians University, Munich, Germany.
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):893-6. doi: 10.1073/pnas.91.3.893.
The monoclonal antibody NEMO is directed against a molecule expressed by human cells of the melanocytic lineage. Although obtained by conventional immunization and fusion procedures, NEMO consists solely of kappa light chain. SDS/PAGE analysis indicates that the kappa chains are present as both monomers and dimers. When these two forms were separated by gel filtration, only the monomeric form bound antigen. As kappa light chains from the myeloma MOPC-41 and the hybridoma MORK do not bind to the melanocytic cells, we conclude that the binding specificity of NEMO resides in the variable region.
单克隆抗体NEMO针对黑素细胞谱系人类细胞所表达的一种分子。尽管NEMO是通过传统免疫和融合程序获得的，但它仅由κ轻链组成。SDS/PAGE分析表明，κ链以单体和二聚体形式存在。当通过凝胶过滤将这两种形式分离时，只有单体形式能结合抗原。由于来自骨髓瘤MOPC - 41和杂交瘤MORK的κ轻链不与黑素细胞结合，我们得出结论，NEMO的结合特异性存在于可变区。
