Orefice G, Campanella G, Cicciarello S, Chirianni A, Borgia G, Rubino S, Mainolfi M, Coppola M, Piazza M
Second Department of Neurology, Medical School, Federico II University, Naples, Italy.
Acta Neurol (Napoli). 1993 Oct;15(5):328-32.
An "in vivo" diagnosis of progressive multifocal leukoencephalopathy (PML), a neurological opportunistic viral infection in AIDS patients, can be made only by brain biopsy. In order to identify viral particles, we examined the cerebrospinal fluid (CSF) of 15 AIDS patients with focal neurological signs by electron microscopy using negative staining technique. In 2 out of 3 patients with clinical and neuroradiological presumptive diagnosis of PML, the CSF examination revealed papova-like viral particles. Our results support the hypothesis that the severe cell-mediated immunodeficiency reactivates papovavirus from a latent state in the brain, leading to PML. Therefore, the CSF study by negative staining might be a useful test for an "in vivo" diagnosis of PML.
进行性多灶性白质脑病（PML）是艾滋病患者的一种神经机会性病毒感染，其“体内”诊断只能通过脑活检来进行。为了鉴定病毒颗粒，我们使用负染色技术，通过电子显微镜检查了15例有局灶性神经体征的艾滋病患者的脑脊液（CSF）。在3例临床和神经放射学初步诊断为PML的患者中，有2例脑脊液检查发现了乳头多瘤空泡病毒样病毒颗粒。我们的结果支持这样一种假说，即严重的细胞介导免疫缺陷使脑中处于潜伏状态的乳头多瘤空泡病毒重新激活，从而导致PML。因此，通过负染色进行脑脊液研究可能是一种用于PML“体内”诊断的有用检测方法。
