Albro P W, Corbett J T
Transfusion. 1978 Nov-Dec;18(6):750-5. doi: 10.1046/j.1537-2995.1978.18679077962.x.
The distributions of di-(2-ethylexyl) phthalate and mono-(2-ethylhexyl) phthalate among the protein constituents of human plasma were studied by selective precipitation and chromatographic procedures. The distributions resulting from the leaching of phthalates from blood bags were simulated conveniently by allowing plasma to extract phthalates from coated Celite. More than 80 per cent of the diester was associated with lipoproteins, in the order: LDL greater than VLDL greater than HDL greater than chylomicrons. The remaining diester was apparently adsorbed weakly and nonspecifically to other proteins, including albumin. Mono-(2-ethylhexyl) phthalate was in equilibrium between free in solution and adsorbed to albumin; none was bound to lipoproteins. Extraction of phthalate from Celite into plasma should provide a convenient preparation for toxicity or pharmacokinetic studies.
通过选择性沉淀和色谱法研究了邻苯二甲酸二（2-乙基己基）酯和邻苯二甲酸单（2-乙基己基）酯在人血浆蛋白质成分中的分布。通过让血浆从涂覆的硅藻土中提取邻苯二甲酸盐，可以方便地模拟血袋中邻苯二甲酸盐浸出产生的分布。超过80%的二酯与脂蛋白结合，顺序为：低密度脂蛋白大于极低密度脂蛋白大于高密度脂蛋白大于乳糜微粒。其余的二酯显然与其他蛋白质（包括白蛋白）发生弱的非特异性吸附。邻苯二甲酸单（2-乙基己基）酯在溶液中的游离态和吸附到白蛋白上之间处于平衡状态；没有与脂蛋白结合。将邻苯二甲酸盐从硅藻土提取到血浆中应该为毒性或药代动力学研究提供一种方便的制剂。
