Sneyd J, Charles A C, Sanderson M J
Department of Biomathematics, School of Medicine, University of California, Los Angeles 90024-1766.
Am J Physiol. 1994 Jan;266(1 Pt 1):C293-302. doi: 10.1152/ajpcell.1994.266.1.C293.
In response to mechanical stimulation of a single cell, intercellular Ca2+ waves propagate through airway epithelial and glial cell cultures, providing a mechanism for intercellular communication. Experiments indicate that intercellular propagation of the Ca2+ wave is mediated by the movement of inositol 1,4,5-trisphosphate (IP3) through gap junctions. To explore the validity of this hypothesis, we have constructed and solved a system of partial differential equations that models the Ca2+ changes induced by the movement of IP3 between cells. The model is in good qualitative agreement with experimental data, including the behavior of the wave in the absence of extracellular Ca2+, the shape of the subsequent asynchronous Ca2+ oscillations, and the passage of a wave through a cell exhibiting Ca2+ oscillations. However, the concentration of IP3 that is required in each cell to propagate the wave may not be achieved by passive diffusion of IP3 through gap junctions from the stimulated cell. We therefore suggest that Ca(2+)-independent regenerative production of IP3 might be necessary for the propagation of intercellular Ca2+ waves.
对单个细胞进行机械刺激时，细胞间的Ca2+波会在气道上皮细胞和神经胶质细胞培养物中传播，这为细胞间通讯提供了一种机制。实验表明，Ca2+波的细胞间传播是由肌醇1,4,5-三磷酸（IP3）通过间隙连接的移动介导的。为了探究这一假说的正确性，我们构建并求解了一个偏微分方程组，该方程组模拟了IP3在细胞间移动所引起的Ca2+变化。该模型在定性上与实验数据高度吻合，包括在无细胞外Ca2+情况下波的行为、随后异步Ca2+振荡的形状以及波通过呈现Ca2+振荡的细胞的过程。然而，通过间隙连接从受刺激细胞被动扩散IP3可能无法在每个细胞中达到传播波所需的IP3浓度。因此，我们认为IP3的Ca(2+)非依赖性再生产生可能是细胞间Ca2+波传播所必需的。
