Lannan S, Donaldson K, Brown D, MacNee W
Department of Medicine, City Hospital and Napier University, Edinburgh, Scotland, United Kingdom.
Am J Physiol. 1994 Jan;266(1 Pt 1):L92-100. doi: 10.1152/ajplung.1994.266.1.L92.
The oxidant-antioxidant balance in the airspaces of the lungs may be critical in protecting the lungs from the effects of cigarette smoke. We studied the effect of cigarette smoke and its condensates on the detachment, attachment, and proliferation of the A549 human alveolar epithelial cell line, in an in vitro model of cell injury and regeneration and the protective effects of antioxidants. Whole and vapor phase cigarette smoke decreased 51Cr-labeled A549 cell attachment, increased cell detachment, and decreased cell proliferation, as assessed by [3H]thymidine uptake. Freshly isolated rat type II alveolar epithelial cells showed an enhanced susceptibility to smoke-induced cell lysis when compared with the A549 cell line. Reduced glutathione (GSH) (400 microM) protected against the effects of cigarette smoke exposure on cell attachment, proliferation, and detachment. Depletion of intracellular GSH with buthionine sulfoxamine enhanced the epithelial cell detachment injury produced by smoke condensates. We conclude that cigarette smoke and its condensates cause an oxidant-induced injury to A549 human type II alveolar epithelial cells. Both intra- and extracellular GSH have important roles in protecting epithelial cells from the injurious effects of cigarette smoke.
肺腔中的氧化还原平衡对于保护肺部免受香烟烟雾的影响可能至关重要。我们在细胞损伤与再生的体外模型中，研究了香烟烟雾及其冷凝物对A549人肺泡上皮细胞系的黏附、脱离和增殖的影响，以及抗氧化剂的保护作用。通过[3H]胸腺嘧啶核苷摄取评估，完整烟雾和气相香烟烟雾降低了51Cr标记的A549细胞黏附，增加了细胞脱离，并降低了细胞增殖。与A549细胞系相比，新鲜分离的大鼠II型肺泡上皮细胞对烟雾诱导的细胞裂解表现出更高的敏感性。还原型谷胱甘肽（GSH）（400微摩尔）可保护细胞免受香烟烟雾暴露对细胞黏附、增殖和脱离的影响。用丁硫氨酸亚砜胺消耗细胞内GSH会增强烟雾冷凝物对上皮细胞脱离的损伤。我们得出结论，香烟烟雾及其冷凝物会导致氧化剂诱导的对A549人II型肺泡上皮细胞的损伤。细胞内和细胞外的GSH在保护上皮细胞免受香烟烟雾的有害影响方面都起着重要作用。
