Krammer H J, Sigge W
Institut für Anatomie und Klinik für Kinderchirurgie, Medizinischen Universität, Lübeck, Deutschland.
Ann Anat. 1994 Jan;176(1):17-21.
A Short bowel was induced in 8 beagle bitches by resecting 85% of the small intestine. Following resection, a tangential fixation was performed in antiperistaltic direction between the ileum and the proximal colon. A 15 cm segment of the remaining ileum was incised opposite to its mesenterial attachment and sewn onto the serosa of the neighboring segment of colon (Serosa patching). After 20 weeks of observation, segments of the ileum, the colon and the anastomosed intestinal segment were removed. The neomucosa covering the patch surface was investigated using immunohistochemical methods in order to demonstrate the presence of nerve fibers. The immunofluorescence reaction using anti-GFAP (glial fibrillary acidic protein) clearly demonstrated immunoreactivity in the formed neomucosa and in the underlying granulation tissue. The immunoreactive fibers were comparable with fibers demonstrated in the original mucosa. This finding is an indication that the neomucosa of a serosa patch may be functionally intact because the presence of nerves suggests that mucosal functions, e.g. resorption and secretion, are under nervous regulation.
通过切除85%的小肠，在8只比格犬母犬中诱导形成短肠。切除术后，在回肠和近端结肠之间沿逆蠕动方向进行切线固定。将剩余回肠的15厘米段在与其肠系膜附着相对的位置切开，并缝合到相邻结肠段的浆膜上（浆膜修补）。观察20周后，切除回肠、结肠和吻合肠段。使用免疫组织化学方法研究覆盖修补表面的新黏膜，以证明神经纤维的存在。使用抗GFAP（胶质纤维酸性蛋白）的免疫荧光反应清楚地显示在形成的新黏膜和下方的肉芽组织中有免疫反应性。免疫反应性纤维与原始黏膜中显示的纤维相当。这一发现表明浆膜修补处的新黏膜可能功能完整，因为神经的存在表明黏膜功能，如吸收和分泌，受神经调节。
