Iwamoto T, Tanaka H, Osada T, Shinagawa T, Osamura Y, Gagnon C
Department of Urology, St. Marianna University School of Medicine, Montreal, Canada.
Mol Reprod Dev. 1993 Dec;36(4):475-81. doi: 10.1002/mrd.1080360411.
We have investigated the origin of the sperm motility inhibitor (SPMI) from boar seminal plasma. SPMI was measured by its capacity to inhibit the motility of demembranated spermatozoa and by an enzyme-linked immunosorbent assay (ELISA). Among the various reproductive and now reproductive tissues and fluids tested, only the seminal vesicle fluid and seminal plasma contained significant amounts of SPMI biological activity and SPMI antigen. Like other seminal vesicle fluid proteins, SPMI is diluted 6- to 8-fold upon ejaculation. By immunohistochemical detection at the light microscope with antibodies obtained from rabbits immunized with SPMI purified from boar seminal plasma, SPMI was found in the cytosol and/or on the plasma membrane bordering the lumen of the seminal vesicles. At the electron microscope level, SPMI appeared to be present only on the surface of the secretory cells. The data indicate that SPMI originates from a single tissue, the seminal vesicle, and suggest that only the mature form present on the luminal surface of the gland can react with the antibody generated from rabbits immunized with the secreted form of SPMI.
我们研究了公猪精浆中精子活力抑制剂（SPMI）的来源。通过其抑制去膜精子活力的能力以及酶联免疫吸附测定（ELISA）来检测SPMI。在测试的各种生殖和非生殖组织及体液中，只有精囊液和精浆含有大量的SPMI生物活性和SPMI抗原。与其他精囊液蛋白一样，射精时SPMI会被稀释6至8倍。用从公猪精浆中纯化的SPMI免疫兔子获得的抗体，在光学显微镜下进行免疫组织化学检测，发现SPMI存在于精囊管腔边界的细胞质和/或质膜上。在电子显微镜水平上，SPMI似乎仅存在于分泌细胞表面。数据表明SPMI起源于单一组织——精囊，并表明只有腺体腔表面存在的成熟形式才能与用分泌形式的SPMI免疫兔子产生的抗体发生反应。
