在精囊中特异性表达并编码精子活力抑制蛋白的公猪SPMI基因的克隆。

Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and codes for a sperm motility inhibitor protein.

作者信息

Iwamoto T, Hiroaki H, Furuichi Y, Wada K, Satoh M, Satoh M, Osada T, Gagnon C

机构信息

Department of Urology, St Marianna University School of Medicine, Kawasaki, Japan.

出版信息

FEBS Lett. 1995 Jul 24;368(3):420-4. doi: 10.1016/0014-5793(95)00701-a.

DOI:10.1016/0014-5793(95)00701-a

PMID:7635190

Abstract

Boar semen contains a seminal plasma motility inhibitor (SPMI) that blocks the motility of demembranated-reactivated spermatozoa as well as of intact spermatozoa. In this paper, we describe the primary structure of SPMI, the coding of boar SPMI cDNA gene and its expression in various porcine tissues. Nucleotide sequence analysis of the 645-bp SPMI cDNA predicts a coded polypeptide of 137 amino acid residues which includes a 21-residue signal peptide and a 116-residue secreted protein. The amino acid sequence of SPMI was found to be highly homologous to AQN-3, a member of spermadhesin family proteins of boar that bind to spermatozoa. Expression of the boar SPMI gene detected by Northern blot analysis revealed that its expression is very abundant in seminal vesicles and specific to this tissue.

摘要

公猪精液中含有一种精液浆运动抑制剂（SPMI），它能抑制去膜再活化精子以及完整精子的运动。在本文中，我们描述了SPMI的一级结构、公猪SPMI cDNA基因的编码及其在各种猪组织中的表达。对645个碱基对的SPMI cDNA进行核苷酸序列分析，预测其编码的多肽有137个氨基酸残基，其中包括一个21个残基的信号肽和一个116个残基的分泌蛋白。发现SPMI的氨基酸序列与AQN-3高度同源，AQN-3是公猪精子黏附素家族蛋白的成员之一，可与精子结合。通过Northern印迹分析检测到的公猪SPMI基因表达表明，其在精囊中表达非常丰富且具有组织特异性。