Porreca E, Ucchino S, Di Febbo C, Di Bartolomeo N, Angelucci D, Napolitano A M, Mezzetti A, Cuccurullo F
Istituto di Patologia Speciale Medica, Università G. D'annunzio Facoltà di Medicina e Chirurgia, Chieti, Italy.
Arterioscler Thromb. 1994 Feb;14(2):299-304. doi: 10.1161/01.atv.14.2.299.
Vascular smooth muscle cell (VSMC) growth is a primary component of accelerated and spontaneous atherosclerosis. Previous studies have shown that iron may be involved in the control of enzymatic activities that modulate DNA synthesis in human cells. In this study the effects of the iron chelator desferrioxamine on in vitro and in vivo VSMC proliferation were tested. Rat VSMCs in culture and a rabbit model of carotid artery balloon injury were used. Desferrioxamine showed a significant inhibitory effect on [3H]thymidine incorporation in cell cultures that was antagonized by iron supplementation. Desferrioxamine also provided effective preventive myointimal VSMC proliferation as assessed by bromodeoxyuridine labeling and morphometric analysis of endoluminal stenosis. These experiments suggested that iron may be involved in the control of VSMC proliferation and that desferrioxamine may have a role in preventing VSMC growth and myointimal proliferative lesions.
血管平滑肌细胞（VSMC）的生长是加速性和自发性动脉粥样硬化的主要组成部分。先前的研究表明，铁可能参与调控调节人类细胞中DNA合成的酶活性。在本研究中，测试了铁螯合剂去铁胺对体外和体内VSMC增殖的影响。使用了培养的大鼠VSMC和颈动脉球囊损伤的兔模型。去铁胺对细胞培养中[3H]胸苷掺入显示出显著的抑制作用，补充铁可拮抗该作用。通过溴脱氧尿苷标记和腔内狭窄的形态计量分析评估，去铁胺还能有效预防肌内膜VSMC增殖。这些实验表明，铁可能参与VSMC增殖的调控，而去铁胺可能在预防VSMC生长和肌内膜增殖性病变中发挥作用。
