Anthony M L, Beddell C R, Lindon J C, Nicholson J K
Department of Chemistry, Birkbeck College, University of London, UK.
J Pharm Biomed Anal. 1993 Oct;11(10):897-902. doi: 10.1016/0731-7085(93)80047-5.
We report the application of spin-echo 1H-NMR spectroscopy to the detection of raised plasma transaminase activity in rats treated with the nephrotoxic cephalosporin antibiotic cephaloridine (CPH). Spin-echo 1H-NMR analysis of lyophilized plasma, reconstituted in H2O reveals a doublet at delta 1.48 for alanine. However when samples were reconstituted with 2H2O we noted that in samples from CPH-treated rats (but not in control samples) there was a variable degree of appearance of a singlet at delta 1.47 together with a reduction in the doublet at delta 1.48. We suggest that this is due to the release of transaminases from damaged tissue which, via a reversible conversion of alanine to pyruvate, causes selective deuteration of alanine at the alpha-hydrogen (alpha-CH) position. This observation suggests that these 1H-NMR spectral patterns are dependent on the level of plasma transaminases and this may provide a novel indicator of tissue damage.
我们报告了自旋回波1H-NMR光谱法在检测用肾毒性头孢菌素抗生素头孢噻啶（CPH）治疗的大鼠血浆转氨酶活性升高方面的应用。对冻干血浆进行自旋回波1H-NMR分析，在重溶于H2O后，丙氨酸在δ1.48处出现双峰。然而，当样品用2H2O重溶时，我们注意到在CPH处理的大鼠样品中（对照样品中未出现），在δ1.47处出现了程度不一的单峰，同时δ1.48处的双峰减少。我们认为这是由于受损组织释放转氨酶，通过丙氨酸向丙酮酸的可逆转化，导致丙氨酸在α-氢（α-CH）位置发生选择性氘代。这一观察结果表明，这些1H-NMR光谱模式取决于血浆转氨酶水平，这可能为组织损伤提供一种新的指标。
