Mascher F, Marth E
Institute of Hygiene University, Graz, Austria.
Cent Eur J Public Health. 1993 Jun;1(1):49-52.
The effect of nitrate on the health of mice in a long term study is described. Nitrate was given to the mice in the form of calcium nitrate in drinking water in varying concentrations, and the mice were observed over the course of 18 months. Three groups were formed: 1. control group, 2. group I (100 mg nitrate/l drinking water) and group II (1,000 mg nitrate/l drinking water). The parameters studied were liver function, kidney function, total iron, ammonium, total protein and electrophoresis of the various serum proteins, body weight, and N-glycolyl-neuraminic acid as a tumor marker. Nitrate is broken down via nitrate, hydroxylamine to ammonium and finally to urea. The concentration of urea increased with time and higher concentrations of nitrate load. The exposed mice clearly lost weight and died prematurely. This was true only for the mice in group II. With a load of 100 mg/l drinking water, no change in the physiological parameters could be seen within the time period studied.
描述了一项长期研究中硝酸盐对小鼠健康的影响。以不同浓度的硝酸钙形式通过饮用水给小鼠喂食硝酸盐，并在18个月的过程中观察这些小鼠。形成了三组：1. 对照组，2. 第一组（100毫克硝酸盐/升饮用水）和第二组（1000毫克硝酸盐/升饮用水）。所研究的参数包括肝功能、肾功能、总铁、铵、总蛋白以及各种血清蛋白的电泳、体重，还有作为肿瘤标志物的N-羟乙酰神经氨酸。硝酸盐通过亚硝酸盐、羟胺分解为铵，最终分解为尿素。尿素浓度随时间和更高的硝酸盐负荷而增加。暴露的小鼠明显体重减轻且过早死亡。这仅在第二组小鼠中出现。对于饮用100毫克/升水的负荷，在所研究的时间段内未观察到生理参数的变化。
