Stockley R A, Grant R A, Llewellyn-Jones C G, Hill S L, Burnett D
Lung Immunobiochemical Research Laboratory, General Hospital, Birmingham, England.
Am J Respir Crit Care Med. 1994 Feb;149(2 Pt 1):464-8. doi: 10.1164/ajrccm.149.2.8306047.
A reproducible assay was established to assess the number of formyl-peptide receptors expressed on the surface of human polymorphonuclear leukocytes (PMN). Using this assay the number of receptors was shown to demonstrate wide within- and between-subject variability. However, the receptor numbers were related to the chemotactic response (r = 0.572) and degranulation response (r = 0.512) to the peptide formyl-methionyl-leucyl-phenylalanine. Subsequent studies showed increased receptor numbers on PMN from patients with emphysema (median, 459 x 10(3)/cell; range, 207 to 1,080) as compared with age-matched control subjects (median, 288; range, 168 to 519; p < 0.02), which may explain the increased chemotactic response of the PMN to formyl peptides. This difference was not observed in patients with bronchiectasis, suggesting that the increased receptor number is a feature of emphysema. Furthermore, the increase was largely a feature of smokers with emphysema (median, 463; range, 362 to 1,080), whereas age-matched smokers without emphysema had lower numbers of receptors (p < 0.001; median, 332; range, 243 to 411). This observation suggests a mechanism that may explain the susceptibility of some smokers to the development of emphysema.
建立了一种可重复的检测方法，以评估人多形核白细胞（PMN）表面表达的甲酰肽受体数量。使用该检测方法，发现受体数量在个体内和个体间存在很大差异。然而，受体数量与对肽甲酰甲硫氨酰亮氨酰苯丙氨酸的趋化反应（r = 0.572）和脱颗粒反应（r = 0.512）相关。随后的研究表明，与年龄匹配的对照受试者相比，肺气肿患者的PMN上的受体数量增加（中位数，459×10³/细胞；范围，207至1080）（中位数，288；范围，168至519；p < 0.02），这可能解释了PMN对甲酰肽的趋化反应增加。在支气管扩张患者中未观察到这种差异，这表明受体数量增加是肺气肿的一个特征。此外，这种增加在很大程度上是肺气肿吸烟者的一个特征（中位数，463；范围，362至1080），而年龄匹配的无肺气肿吸烟者的受体数量较低（p < 0.001；中位数，332；范围，243至411）。这一观察结果提示了一种可能解释一些吸烟者易患肺气肿的机制。
