Yamamoto M
Mol Gen Genet. 1980;180(1):231-4. doi: 10.1007/BF00267375.
Mutants resistant to the antimitotic compounds thiabendazole and methyl-2-benzimidazole-carbamate were isolated and analyzed genetically in the fission yeast. Schizosaccharomyces pombe. They comprised three groups in terms of genetic linkage. Mutants in one linkage group (ben1) differed phenotypically from those in the other two (ben2 and ben3). The former were resistant to the compounds at any physiological temperature tested, whereas the latter exhibited temperature dependent resistance. Through tetrad analysis, ben1 was mapped at the rightmost part of chromosome II, and ben2 was mapped near the centromere of the same chromosome. Haploidization experiments revealed the location of ben3 on chromosome II. By analogy with Aspergillus nidulans, it is suggested that one of these ben genes may code for tubulin.
在裂殖酵母粟酒裂殖酵母中分离出对抗有丝分裂化合物噻苯咪唑和甲基-2-苯并咪唑氨基甲酸酯具有抗性的突变体，并对其进行了遗传分析。根据遗传连锁关系，它们分为三组。一个连锁群（ben1）中的突变体在表型上与其他两个连锁群（ben2和ben3）中的突变体不同。前者在任何测试的生理温度下都对这些化合物具有抗性，而后者表现出温度依赖性抗性。通过四分体分析，ben1被定位在第二条染色体的最右端，ben2被定位在同一条染色体的着丝粒附近。单倍体化实验揭示了ben3在第二条染色体上的位置。通过与构巢曲霉类比，表明这些ben基因中的一个可能编码微管蛋白。
