苏丹内脏利什曼病患者血液中IgM、IgA、IgG及IgG亚类水平的意义
The significance of blood levels of IgM, IgA, IgG and IgG subclasses in Sudanese visceral leishmaniasis patients.
作者信息
Elassad A M, Younis S A, Siddig M, Grayson J, Petersen E, Ghalib H W
机构信息
Department of Microbiology, College of Medicine, University of Juba.
出版信息
Clin Exp Immunol. 1994 Feb;95(2):294-9. doi: 10.1111/j.1365-2249.1994.tb06526.x.
We developed an ELISA test using leishmania antigenic extracts to detect antigen-specific antibody responses, including subclass and isotype analysis, in visceral leishmaniasis (VL) patients from the Sudan. A total of 92 parasitologically proven patients were compared with cutaneous leishmaniasis, schistosomiasis, malaria, onchocerciasis and tuberculosis patients, as well as with healthy endemic and non-endemic controls. Some VL patients were examined before and after chemotherapy. VL patients showed significantly higher IgG responses compared with all other groups (93.4% sensitivity, 93.7% specificity), and higher (but not significantly) IgM responses. All groups showed low IgA levels. All IgG subclasses, IgG1, 2, 3, and 4, showed higher levels in patients than all other groups, with IgG1 and IgG3 levels being significantly reduced following treatment. The rank order for specificity and sensitivity for IgG subclasses was IgG3 > IgG1 > IgG2 > IgG4.
我们开发了一种酶联免疫吸附测定（ELISA）试验，使用利什曼原虫抗原提取物来检测苏丹内脏利什曼病（VL）患者的抗原特异性抗体反应，包括亚类和亚型分析。总共92例经寄生虫学证实的患者与皮肤利什曼病、血吸虫病、疟疾、盘尾丝虫病和结核病患者，以及健康的流行区和非流行区对照进行了比较。一些VL患者在化疗前后接受了检查。与所有其他组相比，VL患者表现出显著更高的IgG反应（敏感性93.4%，特异性93.7%），以及更高（但不显著）的IgM反应。所有组的IgA水平都很低。所有IgG亚类，即IgG1、2、3和4，在患者中的水平均高于所有其他组，治疗后IgG1和IgG3水平显著降低。IgG亚类的特异性和敏感性排序为IgG3 > IgG1 > IgG2 > IgG4。
Zotero 插件