MacCalman C D, Brodt P, Doublet J D, Jednak R, Elhilali M M, Bazinet M, Blaschuk O W
Department of Surgery, McGill University, Royal Victoria Hospital, Montreal, Quebec, Canada.
Clin Exp Metastasis. 1994 Mar;12(2):101-7. doi: 10.1007/BF01753976.
We compared the levels of mRNA transcripts encoding E-cadherin, N-cadherin, beta 1 integrin subunit, alpha 5 integrin subunit and fibronectin in the normal rat prostate gland, as well as in tumors derived from three invasive sublines (G, MatLyLu, AT-2) of the Dunning R-3227 rat prostatic adenocarcinoma. E-cadherin mRNA transcripts were only detectable in total RNA extracts prepared from normal rat prostates, whereas N-cadherin mRNA transcripts were only found in normal rat brains. In contrast, the mRNA transcripts encoding the beta 1 integrin subunit, alpha 5 integrin subunit and fibronectin were all elevated in the tumors, as compared to the levels of these transcripts in normal tissues. Our results suggest that there is an inverse correlation between cadherin and integrin mRNA levels in rat prostatic tumors.
我们比较了正常大鼠前列腺以及源自邓宁R-3227大鼠前列腺腺癌的三个侵袭性亚系（G、MatLyLu、AT-2）肿瘤中编码E-钙黏蛋白、N-钙黏蛋白、β1整合素亚基、α5整合素亚基和纤连蛋白的mRNA转录物水平。E-钙黏蛋白mRNA转录物仅在从正常大鼠前列腺制备的总RNA提取物中可检测到，而N-钙黏蛋白mRNA转录物仅在正常大鼠脑中发现。相反，与正常组织中这些转录物的水平相比，编码β1整合素亚基、α5整合素亚基和纤连蛋白的mRNA转录物在肿瘤中均升高。我们的结果表明，大鼠前列腺肿瘤中钙黏蛋白和整合素mRNA水平之间存在负相关。
