Glasgow B J, Heinzmann C, Kojis T, Sparkes R S, Mohandas T, Bateman J B
Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024.
Curr Eye Res. 1993 Nov;12(11):1019-23. doi: 10.3109/02713689309029229.
We assigned the gene for tear lipocalin to the long arm of human chromosome 9. Polyadenylated RNA was extracted from lacrimal gland. The coding region for tear lipocalin was amplified, sequenced and used to probe a panel of somatic cell hybrid DNA by Southern blot analysis. Regional mapping was accomplished by probing a panel of subfragments of the indicated chromosome. Restriction of genomic DNA with EcoRI failed to reveal any bands corresponding to the human tear lipocalin gene in mouse-human hybrids all of which lack chromosome 9. Southern blot analysis of human-hamster hybrids demonstrated a human 5.6 kb TaqI restriction fragment that segregated to the q34-qter region of chromosome 9 and assigned the gene for tear lipocalin to this region. Structurally homologous proteins of the lipocalin family, human placental protein 14, human alpha 1 microglobulin, and human brain prostaglandin synthase, have been mapped to this region. We suggest that the gene for tear lipocalin is part of an important lipocalin superfamily gene cluster on chromosome 9 within band q34.
我们将泪液脂质运载蛋白基因定位于人类9号染色体长臂。从泪腺中提取多聚腺苷酸化RNA。扩增泪液脂质运载蛋白的编码区，进行测序，并通过Southern印迹分析用于探测一组体细胞杂种DNA。通过探测指定染色体的一组亚片段完成区域定位。用EcoRI限制基因组DNA未能在所有缺失9号染色体的小鼠-人类杂种中揭示任何与人类泪液脂质运载蛋白基因相对应的条带。对人类-仓鼠杂种的Southern印迹分析显示一个5.6 kb的人类TaqI限制片段，该片段定位于9号染色体的q34-qter区域，并将泪液脂质运载蛋白基因定位于该区域。脂质运载蛋白家族的结构同源蛋白，人类胎盘蛋白14、人类α1微球蛋白和人类脑前列腺素合酶，已被定位于该区域。我们认为泪液脂质运载蛋白基因是9号染色体q34带内一个重要的脂质运载蛋白超家族基因簇的一部分。
