Lai Z, Zhai Q, Yukhananov R, Larsson C, Roos P, Kreek M J, Nyberg F
Department of Pharmacology, University of Uppsala, Sweden.
FEBS Lett. 1994 Jan 31;338(2):207-11. doi: 10.1016/0014-5793(94)80366-8.
The effect of chronically given morphine on the binding of ovine prolactin (oPRL) to specific areas in the male rat brain was studied. The drug was delivered through subcutaneously implanted miniosmotic pumps. The results indicated that the density of prolactin binding sites in the hypothalamus and the choroid plexus was significantly decreased in the acute phase of morphine administration but restored to control levels when tolerance to morphine was developed. The decrease in prolactin binding was contrasted by elevated plasma levels of the hormone. A negative correlation was found between the hormone concentration in plasma and the density of its binding sites in the hypothalamus and choroid plexus. The hormone-binding sites in these two regions were further characterized with regard to binding constants and molecular sizes. The relevance of the present results with respect to the hypothalamic control of prolactin secretion is discussed.
研究了长期给予吗啡对雄性大鼠脑内特定区域绵羊催乳素（oPRL）结合的影响。药物通过皮下植入的微型渗透泵给药。结果表明，在吗啡给药急性期，下丘脑和脉络丛中催乳素结合位点的密度显著降低，但在产生吗啡耐受性时恢复到对照水平。催乳素结合的减少与该激素血浆水平升高形成对比。发现血浆中激素浓度与其在下丘脑和脉络丛中结合位点密度之间呈负相关。对这两个区域的激素结合位点在结合常数和分子大小方面进行了进一步表征。讨论了本研究结果与下丘脑对催乳素分泌控制的相关性。
