Huang C F, Schmidt J
Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794.
FEBS Lett. 1994 Feb 7;338(3):277-80. doi: 10.1016/0014-5793(94)80283-1.
The transcriptional activity of the acetylcholine receptor alpha-subunit gene was measured in denervated chick skeletal muscle in response to calcium-active drugs, using a ribonuclease protection version of the conventional run-off assay. The L-channel agonist (-)Bay-K6844 and the calcium ionophore A23187 mimicked, and the intracellular chelator BAPTA and the calcium channel blockers D600 and nifedipine blocked, the effect of electrostimulation. These results suggest that influx of extracellular calcium is an integral component of the membrane depolarization-receptor gene inactivation cascade.
利用传统的连续转录分析的核糖核酸酶保护法，检测了失神经支配的鸡骨骼肌中乙酰胆碱受体α亚基基因对钙活性药物的转录活性。L型通道激动剂（-）Bay-K6844和钙离子载体A23187模拟了电刺激的作用，而细胞内螯合剂BAPTA以及钙通道阻滞剂D600和硝苯地平则阻断了电刺激的作用。这些结果表明，细胞外钙的内流是膜去极化-受体基因失活级联反应的一个组成部分。
