茄子下胚轴中编码属于一个新家族（CYP77）细胞色素P450的cDNA的克隆。
Cloning of eggplant hypocotyl cDNAs encoding cytochromes P450 belonging to a novel family (CYP77).
作者信息
Toguri T, Tokugawa K
机构信息
Central Laboratory for Key Technology, Kirin Brewery Co. Ltd, Yokohama, Japan.
出版信息
FEBS Lett. 1994 Feb 7;338(3):290-4. doi: 10.1016/0014-5793(94)80286-6.
From eggplant hypocotyl tissues we have cloned two closely related cDNAs encoding cytochromes P450 (P450s) by PCR amplification using a primer designed based on the highly conserved sequence among the known eggplant P450s. One cDNA lacks the NH2-terminal short sequence that is present in the other, full-length cDNA. The two predicted protein sequences are 71% identical with each other and show less than 30% identity with any other known P450s. It is concluded that these P450s, which are termed CYP77A1 and -A2, belong to a hitherto unknown P450 family.
我们从茄子下胚轴组织中，使用基于已知茄子细胞色素P450（P450s）中高度保守序列设计的引物，通过PCR扩增克隆了两个密切相关的编码细胞色素P450的cDNA。一个cDNA缺少另一个全长cDNA中存在的NH2末端短序列。这两个预测的蛋白质序列彼此有71%的同一性，并且与任何其他已知的P450s的同一性低于30%。得出的结论是，这些被称为CYP77A1和 -A2的P450s属于一个迄今未知的P450家族。
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