McCormick M K, Buckler A, Bruno W, Campbell E, Shera K, Torney D, Deaven L, Moyzis R
Center for Human Genome Studies and Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Genomics. 1993 Dec;18(3):553-8. doi: 10.1016/s0888-7543(05)80355-6.
Human chromosome 9 DNA, flow-sorted from somatic cell hybrid PK-87-9, has been used to construct two complete digest YAC libraries. The combined representation of chromosome 9 in these libraries, estimated by hybridization of chromosome 9-specific sequences to YAC colony grids, is approximately 95%. The frequency of chimeric clones, analyzed by fluorescence in situ hybridization of chromosome 9 YACs to human metaphase chromosomes, was estimated to be approximately 4%. These libraries provide a resource for physical mapping and for moving from genetic markers to disease loci on chromosome 9.
从体细胞杂种PK - 87 - 9中分选出的人类9号染色体DNA已被用于构建两个完全酶切的酵母人工染色体（YAC）文库。通过9号染色体特异性序列与YAC菌落网格杂交估计，这些文库中9号染色体的综合覆盖率约为95%。通过将9号染色体YAC与人类中期染色体进行荧光原位杂交分析，嵌合克隆的频率估计约为4%。这些文库为9号染色体的物理图谱构建以及从遗传标记定位到疾病基因座提供了资源。
