环磷酸腺苷抑制巨噬细胞的抑制功能并增强淋巴细胞增殖。
Cyclic AMP inhibits macrophage suppressor function and enhances lymphocyte proliferation.
作者信息
Gonsalkorale W M, Dascombe M J, Hutchinson I V
机构信息
School of Biological Sciences, University of Manchester, U.K.
出版信息
Immunology. 1993 Dec;80(4):611-6.
The effects of increasing the level of cyclic AMP (cAMP) activity on lymphocyte proliferation in the rat mixed lymphocyte reaction (MLR) were investigated. Dibutyryl cAMP (dbcAMP) and prostaglandin E2 (PGE2) produced a dose-dependent reduction in proliferation in the lymph node (LN) MLR, but produced a substantial increase in proliferation in the spleen MLR at the lower concentrations used (10(-5)-10(-4) M dbcAMP; 10(-7)-10(-6) M PGE2). Enhancement of proliferation was dependent on the presence of macrophages and was probably due to inhibition of macrophage activation. This was based on the following findings: (1) spleen MLR proliferation was lower than that in the LN MLR; (2) depletion of spleen macrophages increased proliferation in the spleen MLR and addition of these macrophages to the LN MLR reduced proliferation; (3) macrophage depletion from the spleen MLR abolished the proliferation-enhancing effect of dbcAMP. In conclusion, cAMP enhances lymphocyte proliferation in this system, apparently as a consequence of suppressing the inhibitory influence of macrophages.
研究了提高环磷酸腺苷（cAMP）活性水平对大鼠混合淋巴细胞反应（MLR）中淋巴细胞增殖的影响。二丁酰环磷腺苷（dbcAMP）和前列腺素E2（PGE2）在淋巴结（LN）MLR中可产生剂量依赖性的增殖降低，但在较低浓度（10^(-5)-10^(-4)M dbcAMP；10^(-7)-10^(-6)M PGE2）下，可使脾脏MLR中的增殖显著增加。增殖增强依赖于巨噬细胞的存在，可能是由于巨噬细胞活化受到抑制。这基于以下发现：（1）脾脏MLR增殖低于LN MLR；（2）去除脾脏巨噬细胞可增加脾脏MLR中的增殖，而将这些巨噬细胞添加到LN MLR中则降低增殖；（3）从脾脏MLR中去除巨噬细胞消除了dbcAMP的增殖增强作用。总之，在该系统中，cAMP增强淋巴细胞增殖，显然是抑制巨噬细胞抑制作用的结果。
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