Ohkubo T, Kudo M, Sugawara K
Department of Pharmacy, Hirosaki University Hospital, Japan.
J Chromatogr. 1992 Jan 17;573(2):289-93. doi: 10.1016/0378-4347(92)80131-9.
The chromatographic behaviour of ofloxacin on various sorbents, including ODS, C8, C1, nitril, phenyl and tert,-butyl, as stationary phases was investigated and a high-performance liquid chromatography (HPLC) assay was developed for the determination of ofloxacin in serum. The serum samples were directly introduced onto an HPLC column after filtering through a Morcut II membrane filter to remove proteins. The filtrate was concentrated on a pre-column using a phenyl stationary phase and was then introduced to an analytical column with an ODS stationary phase by column switching. Ofloxacin and enoxacin as an internal standard were detected by ultraviolet absorbance at 300 nm. Determination was possible for ofloxacin over the concentration range 50-2000 ng/ml; the limit of detection was 20 ng/ml. The recovery of ofloxacin added to serum was 88.8-101.7% with a coefficient of variation of less than 5.2%. This method is applicable to pharmacokinetic studies of patients after treatment with ofloxacin.
研究了氧氟沙星在包括ODS、C8、C1、腈基、苯基和叔丁基等各种吸附剂作为固定相时的色谱行为，并开发了一种高效液相色谱（HPLC）法用于测定血清中的氧氟沙星。血清样品经Morcut II膜滤器过滤以去除蛋白质后，直接进样到HPLC柱上。滤液在预柱上使用苯基固定相进行浓缩，然后通过柱切换引入到具有ODS固定相的分析柱中。氧氟沙星和作为内标的依诺沙星通过300 nm处的紫外吸光度进行检测。氧氟沙星在50 - 2000 ng/ml的浓度范围内均可测定；检测限为20 ng/ml。添加到血清中的氧氟沙星回收率为88.8 - 101.7%，变异系数小于5.2%。该方法适用于氧氟沙星治疗后患者的药代动力学研究。
