用于测定大鼠胚胎培养液中前列腺素E1和E2的液相色谱和放射免疫测定法。

Liquid chromatography and radioimmunoassay method for the determination of prostaglandins E1 and E2 in rat embryo incubates.

作者信息

Hotter G, Roselló-Catafau J, Closa D, Bioque G, Gelpí E, Javerbaum A, González E, Gimeno M A

机构信息

Molecular Pathology Unit, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.

出版信息

J Chromatogr. 1993 Nov 26;655(1):85-8. doi: 10.1016/0021-9673(93)87014-d.

DOI:10.1016/0021-9673(93)87014-d

PMID:8308097

Abstract

This paper describes the application of a combined high-performance liquid chromatography and radioimmunological assay method for the measurement of prostaglandins E1(PGE1) and E2(PGE2). Samples were acidified to pH 3.15, extracted twice with ethyl acetate and further processed through C18 solid-phase extraction cartridges. After HPLC purification, PGE1 and PGE2 were measured by radioimmunological techniques. The limit of detection for PGE1 was 3.9 pg/ml and the intra-assay relative standard deviation was 7.8% for n = 5. The accuracy of the assay procedure was also verified. The method has been applied to the determination of PGE1 and PGE2 in embryo incubates from 10-day pregnant rats.

摘要

本文描述了一种结合高效液相色谱法和放射免疫分析法用于测量前列腺素E1（PGE1）和E2（PGE2）的方法。将样品酸化至pH 3.15，用乙酸乙酯萃取两次，并通过C18固相萃取柱进一步处理。经过高效液相色谱纯化后，采用放射免疫技术测量PGE1和PGE2。PGE1的检测限为3.9 pg/ml，n = 5时批内相对标准偏差为7.8%。该分析方法的准确性也得到了验证。该方法已应用于测定孕10天大鼠胚胎培养液中的PGE1和PGE2。