Kawano J, Ide S, Oinuma T, Suganuma T
Department of Anatomy, Miyazaki Medical College, Japan.
J Histochem Cytochem. 1994 Mar;42(3):363-9. doi: 10.1177/42.3.8308253.
We have produced a new protein-specific monoclonal antibody (MAb) to rat liver beta 1-->4 galactosyltransferase. This MAb, GTL2, was selected as the most reactive IgG to a periodate-treated antigen. Antigen and protein specificities of GTL2 were verified by immunoblotting of a non-glycosylated recombinant protein of human galactosyltransferase and enzymatically deglycosylated rat galactosyltransferase. Using GTL2, an immunohistochemical study was done in rat liver, epididymis, and salivary glands. Intense staining was observed in Golgi areas of epididymal duct epithelial cells, and submandibular and sublingual acinar cells. Hepatocytes showed weaker staining. Immunoelectron microscopic observation revealed that the staining was exclusively localized in trans-Golgi membranes of these cells.
我们制备了一种针对大鼠肝脏β1→4半乳糖基转移酶的新型蛋白特异性单克隆抗体（MAb）。这种单克隆抗体GTL2被选为对经高碘酸盐处理的抗原有最强反应性的IgG。通过对人半乳糖基转移酶的非糖基化重组蛋白和经酶法去糖基化的大鼠半乳糖基转移酶进行免疫印迹，验证了GTL2的抗原和蛋白特异性。使用GTL2，在大鼠肝脏、附睾和唾液腺中进行了免疫组织化学研究。在附睾管上皮细胞、下颌下腺和舌下腺腺泡细胞的高尔基体区域观察到强烈染色。肝细胞染色较弱。免疫电子显微镜观察显示，染色仅定位于这些细胞的反式高尔基体膜上。
