Harwig S L, Harwig J F, Sherman L A, Coleman R E, Welch M J
J Nucl Med. 1977 Jan;18(1):42-5.
We have reinvestigated radioiodinated plasminogen as an agent for localizing preformed thrombi. Canine plasminogen was isolated from fresh plasma by the affinity chromatography technique on a lysine-sepharose 4B column and tagged with I-123 or I-131, at less than one iodine atom per molecule of enzyme, by the conventional ICI method. When injected into dogs more than 2 days after thrombus induction, radioiodinated plasminogen produced thrombus-to-blood activity ratios of 7.8 +/- 2.4. Thrombi as old as 6 days can be visualized in 80% of the cases. Both the weight of the thrombus and the thrombus-to-blood ratio are more variable for 1-day-old thrombi; this may be associated with plasminogen release accompanying thrombus retraction. The results suggest that radioiodinated plasminogen has potential as an imaging agent for pre-existing thrombi.
我们重新研究了放射性碘化纤溶酶原作为定位预先形成血栓的一种试剂。通过在赖氨酸-琼脂糖4B柱上的亲和层析技术从新鲜血浆中分离犬纤溶酶原，并采用传统的ICI方法，以每分子酶少于一个碘原子的量用I-123或I-131进行标记。在诱导血栓形成超过2天后注入犬体内时，放射性碘化纤溶酶原产生的血栓与血液活性比为7.8±2.4。在80%的病例中，6天龄的血栓都可以被显影。对于1天龄的血栓，血栓重量和血栓与血液的比例变化更大；这可能与血栓退缩伴随的纤溶酶原释放有关。结果表明，放射性碘化纤溶酶原具有作为已存在血栓成像剂的潜力。
