Garn H, Krause H, Enzmann V, Drössler K
Department of Biosciences, University of Leipzig, Germany.
J Immunol Methods. 1994 Feb 10;168(2):253-6. doi: 10.1016/0022-1759(94)90062-0.
A modification of the MTT based tetrazolium colorimetric assay is described. Using the electron-coupling agent menadione formazan formation by murine splenocytes and P-815 cells was significantly increased whereas dye reduction by macrophages was hardly influenced. These observations suggest that it should be possible to improve the tetrazolium based cytotoxicity assays of murine macrophages against cells of the syngeneic tumour cell line P-815.
描述了一种基于MTT的四唑盐比色法的改良方法。使用电子偶联剂甲萘醌时，小鼠脾细胞和P - 815细胞形成甲臜的量显著增加，而巨噬细胞对染料的还原作用几乎没有受到影响。这些观察结果表明，有可能改进基于四唑盐的小鼠巨噬细胞对同基因肿瘤细胞系P - 815细胞的细胞毒性测定方法。
